Journal: Biosensors
Article Title: Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips.
doi: 10.3390/bios13070733
Figure Lengend Snippet: Figure 8. Transmitted light microscopy images of red blood cells (RBCs) (a) A meandering microchan- nel where whole blood is loaded. The microchannel has a dark contrast due to the high concentration of red blood cells (RBCs) in the whole blood. (b) A magnified area of the red dotted region in (a). (c) A magnified area of the red dotted region in (b). Individual RBCs are observed. A large num- ber of RBCs contribute a dark contrast (almost black contrast) in the middle of the microchannel. (d,f,h,j) Optical density (OD) characterizations for hemagglutination reactions of whole blood A, B, O, and AB with anti-A and anti-B both with and without aperture in a 3D microtrap chip. Low OD values imply agglutinations, while high OD values imply non-agglutinations. (e,g,i,k) Transmitted light microscopy images of 3D microtrap chips after hemagglutination reactions were induced by whole blood A, B, O, and AB, with Anti-A and Anti-B. Bright channels imply agglutination, while dark channels imply non-agglutination.
Article Snippet: For transmitted light microscopy observations, we used conventional inverted microscopy (Leica DMi8, Thunder imager, Wetzlar, Germany) for large-area imaging.
Techniques: Light Microscopy, Concentration Assay, Agglutination